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Figure 4 | BMC Molecular Biology

Figure 4

From: Mutations in the Lactococcus lactis Ll.LtrB group II intron that retain mobility in vivo

Figure 4

Schematic representation of donor and recipient constructs and the homing product. The donor construct (chloramphenicol resistant – camR) expresses the ΔORF version of Ll.LtrB that carries a T7 promoter in DIV. LtrA is expressed downstream of the intron. The recipient plasmid (ampR) carries ligated exons E1 and E2 – the target site for Ll.LtrB insertion, upstream of a promoterless tetRgene. E1 and E2 are flanked by E. coli rrn B terminators T1 and T2 to prevent leaky expression of tetR. The T7 terminator TΦ is located downstream of the tetRgene. The homing product depicts Ll.LtrB inserted into the target site.

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