Figure 9
Mutational analysis of the COX-2 promoter in NB-4 neuroblastoma cells. The activity of a COX-2 promoter construct containing both NF-κB binding sites was set to 100 %. A construct containing the same promoter context but a mutation of the promoter distal NF-κB1 binding site (κB1mut) had only one tenth of wild-type COX-2 promoter activity. These data show that the constitutive NF-κB activity of the COX-2 promoter in NB-4 cells is merely dependent on the functional integrity of the evolutionary conserved NF-κB1 element. Standard deviations of triplicate transfection assays are depicted as arrow bars.