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Figure 7 | BMC Molecular Biology

Figure 7

From: Cyclooxygenase-2 is a neuronal target gene of NF-κB

Figure 7

Analysis of NF-κB dependent promoter activation in NB-4 neuroblastoma cells. (A) Analysis of a synthetic NF-κB-dependent reporter. The synthetic NF-κB-dependent reporter-gene tk(NF-κB)6-luc was transfected in NB-4 neuroblastoma cells (1). Luciferase activity of tk(NF-κB)6-luc set to 100 %. Cotransfection of tk(NF-κB)6-luc with an expression vector for IκBα totally abrogated the activity of this luciferase reporter (2). Treatment of cells transfected with tk(NF-κB)6-luc with PMA (3) or TNFα (5) resulted in a reduction of reporter gene activity. This residual activity could be further inhibited after expression of IκBα (4). (B) Analysis of the human COX-2 promoter. The COX-2-promoter-driven luciferase reporter was transfected in NB-4 neuroblastoma cells (1). Luciferase activity of COX-2-luc was set to 100 %. Cotransfection of COX-2-luc with an expression vector for IκBα significantly inhibited the activity of this luciferase reporter (2). Treatment of cells transfected with COX-2-luc with PMA (3) resulted in a slight increase of reporter gene activity. This residual activity also could be inhibited after expression of IκBα (4). Standard deviations of triplicate transfection assays are depicted as arrow bars.

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