Figure 3

NF-κB and COX-2 are repressed by aspirin in cultivated hippocampal slices. Left panel: Control (Con) cultures are treated with bicuculline and picrotoxine to influence GABAergic input. Under these conditions NF-κB activity and COX-2 expression is similar to the in vivo situation (see Fig. 2). Right panel: cultures were treated with bicuculline and picrotoxine as control cultures and co-treated with aspirin for3 15 min and analyzed 6 h post treatment.3 Note the strong inhibition of nuclear NF-κB immunoreactivity and the correlated down regulation of COX-2 expression. (× 400)