Aggregation and retention of human urokinase type plasminogen activator in the yeast endoplasmic reticulum

BMC Molecular Biology

Table 2 Comparison of secretion efficiencies of different uPA variants by H. polymorpha

	Amount in culture medium determined by:
uPA variant	Fibrinolytic activity assay (%)	Western blotting* (%)
uPA	100	100
uPA-C	1850	1500
uPA-Q	30	14
uPA-CQ	770	330

*Calculated by comparison of intensities of the 30 kDa band revealed by Western blotting of serially diluted culture supernatants. uPA in culture medium was treated with EndoH prior to the analysis.

ISSN: 1471-2199