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Table 1 Dependence of u-PA levels on the copy number and integration locus of the expression cassette.

From: Aggregation and retention of human urokinase type plasminogen activator in the yeast endoplasmic reticulum

 

Copy number in locus:

u-PA (%)

Strain

mox

leu2

telomere

activity in culture medium

activity in cell lysate

amount in cell lysate*

DLU/L

1

  

100

100

100

DLΔM/pAM226

 

1

 

96

119

98

DLΔM/pAM219/1

  

1

68

59

21

DLΔM/pAM219/2

  

2

50

49

170

DLU/pAM219

1

 

1

29

71

560

DLU/pAM226

1

1

 

17

45

620

DLΔM/pAM281/1

  

~8

10

7

1400

DLΔM/pAM281/2

  

~8

20

7

1100

  1. *Calculated by comparison of intensities of the 50 kDa band revealed by Western blotting of serially diluted cell lysates.
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BMC Molecular Biology

ISSN: 1471-2199

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