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Figure 1 | BMC Molecular Biology

Figure 1

From: Aggregation and retention of human urokinase type plasminogen activator in the yeast endoplasmic reticulum

Figure 1

Schematic representation of the uPA variants (A) and Western blot analysis of uPA in subcellular fractions of transformants expressing different uPA variants (B). Arrows indicate the position of activation cleavage site."Q302" indicates the mutation abolishing the N-glycosylation of uPA. Transformants DLU, DLC, DLQ and DLCQ, expressing uPA, uPA-C, uPA-Q and uPA-CQ, respectively, are described in Materials and methods. P and S, pellet and supernatant fractions, respectively. Pellets were dissolved in a 2-fold volume of starting lysate.

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