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Figure 7 | BMC Molecular Biology

Figure 7

From: Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNA structures

Figure 7

Sedimentation analysis of *VLF-1. Purified *VLF-1 (10 μg in 60 μl) was layered over a 15 to 30% glycerol gradient and centrifuged in an SW 50.1 rotor at 48,000 rpm and 4°C for 22 h. The gradient was fractionated from the bottom into 14 fractions, and 5-μl portions from each fraction were analyzed by SDS-10% PAGE followed by silver staining. The position of the standards centrifuged in separate tubes is shown by arrows: BSA, 66 kDa, 4.3 S (fraction 8.45) and ovalbumin, 45 kDa, 3.55 S (fraction 9.86). The position of *VLF-1 (fraction 9.25) is indicated by the asterisk.

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