Skip to main content
Figure 6 | BMC Molecular Biology

Figure 6

From: Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNA structures

Figure 6

Hydrolysis of different DNA conformations with phage T4 endonuclease VII. Reaction mixtures (10 μl) containing 0.004 pmol 32P-labeled oligonucleotide probe, 50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 15% glycerol, 100 μg of BSA per ml, and 2 mM DTT were incubated for 20 min at 37°C in the presence of phage T4 endonuclease VII (20 units) (lanes 2, 4, 6, 8, and 10) or in its absence (lanes 1, 3, 5, 7, and 9). The reactions were terminated and the samples were processed for electrophoresis in a native 6% polyacrylamide gel as described in Materials and Methods. Reactions shown in lanes contained the following DNA probes: lanes 1 and 2, linear single-stranded DNA (substrate I); lanes 3 and 4, linear double-stranded DNA (substrate II); lanes 5 and 6, forked DNA (pseudo-Y form, substrate III); lanes 7 and 8, three-way junction DNA (substrate IV); and lanes 9 and 10, cruciform DNA (X-shaped form, substrate V).

Back to article page