Figure 2
From: Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNA structures
Binding of *VLF-1 to different DNA conformations as determined by electrophoretic mobility shift assays. Reaction mixtures (10 μl) containing 0.004 pmol 32P-labeled oligonucleotide probe and increasing amounts of *VLF-1 were incubated for 20 min at 23°C, fixed with 0.5% glutaraldehyde, and analyzed by electrophoresis in a native 6% polyacrylamide gels as described in Materials and Methods. Reactions shown in lanes contained the following DNA probes: lanes 1 to 4, linear single-stranded DNA (substrate I); lanes 5 to 8, linear double-stranded DNA (substrate II); lanes 9 to 12, forked DNA (pseudo-Y form, substrate III); lanes 13 to 16, three-way junction DNA (substrate IV); and lanes 17 to 20, cruciform DNA (X-shaped form, substrate V). The following amounts of *VLF-1 were added to the reaction mixtures: lanes 2, 6, 10, 14, and 18, 12.5 ng; lanes 3, 7, 11, 15, and 19, 25 ng; lanes 4, 8, 12, 16, and 20, 50 ng. No protein was added to the reactions shown in lanes 1, 5, 9, 13, and 17. The position of a complex of *VLF-1 with cruciform DNA is indicated by asterisk.