Figure 4From: Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell lineWestern Blot analysis of tTA expression in two independent pools of TRE-tTA infected cells. The cells were induced for 72 hours. In pools 1 and 2, tTA is expressed in a tetracycline-dependent manner, demonstrating that the system functions in an autoregulatory manner. β-catenin is used as a loading control.Back to article page