Figure 2From: Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell lineGeneration and analysis of modified RevTetOFF constructs. (A) Three additional constructs were generated in which the CMV promoter was replaced by either the elongation factor 1-alpha or phosphoglycerate kinase-1 promoters, or a tetracycline responsive element. (B) Parental HC11 cells were transiently co-transfected with the indicated constructs and maintained for 48 hours in the presence of absence of 1 μg/ml doxycycline. The cells were fixed and stained for β-galactosidase expression using X-gal 48 hours after transfection.Back to article page