Figure 5
From: Loss of cellular adhesion to matrix induces p53-independent expression of PTEN tumor suppressor
Phosphorylation status of focal adhesion kinase in suspension cells MDAH cells were transferred to suspension culture conditions for the times indicated. Total cellular lysate was prepared and analyzed by Western blot with antibodies specific for the tyrosine-phosphorylated form of focal adhesion kinase (p-tyr-FAK) or for the overall levels of focal adhesion kinase protein (FAK) as indicated.