Figure 3From: Loss of cellular adhesion to matrix induces p53-independent expression of PTEN tumor suppressorPTEN mRNA transcription in suspension culture cells MDAH cells were transferred to suspension culture conditions for 12 hours. To analyze the transcription of the PTEN gene, nuclei were harvested and nuclear run-off analysis was performed essentially as described [64]. As a control, the radiolabeled RNA was also hybridized to a DNA sequence representing the choA gene, as well as to non-gene-specific sequences from the plasmid pBluescript (pBs).Back to article page