Figure 2

PTEN mRNA levels in suspension culture cells MDAH or 10T1/2 cells were transferred to suspension culture conditions for the times indicated. Poly A⁺ RNA was harvested and subjected to Northern blot analysis. To detect PTEN mRNA, a radioactively labeled PTEN cDNA fragment was used. To control for the amounts of mRNA loaded in each lane, the filters were stripped and rehybridized to a probe for β-actin, as well as a probe for choA.