Figure 1
USF isoforms bind to the RIIβ E-box. A [32P]-labelled oligonucleotide from the RIIβ promoter region (-305 to -268) was incubated with Sertoli cell nuclear extracts and subjected to EMSA. Supershift experiments were performed with 2 μl of antiserum against USF1 N-terminal region (lane 2), USF2a C-terminal sequences (lane 3), or USF2 N-terminal sequences (lane 4).