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Figure 4 | BMC Molecular Biology

Figure 4

From: Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae

Figure 4

Overexpression of eRF1 and N-terminal truncation of eRF3 inhibits nonsense suppressor phenotype of transformants with multicopy ITT1 plasmid. The strain 5V-H19 harboring pairs of plasmids YEplac181-ITTl/YEplacl95, YEplac181/YEplac195, YEplacl81- ITTl/YEplacl95-SUP45 or YEplacl81-ITTl/YEplacl95-SUP35 and the strain 1-5V-H19 (SUP35-C) with YEplac181- ITT1/YEplac19 5 or YEplac181/YEplac195 were grown on medium selective for plasmids, patched on SC-Ade and SC+Ade plates and incubated for three days. The growth of three independently obtained transformants of each strain is shown.

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