Figure 3From: Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiaeItt1p interacts with both eRF3 and eRF1. All proteins were isolated from E. coli, except eRF3 (S.c.), isolated from S. cerevisiae. The indicated proteins were incubated with GST-Itt1p or GST proteins immobilized on glutathione-Sepharose 4B. Following washing, bound proteins were eluted and analyzed by Western blotting with polyclonal antibodies against eRF3 and eRF1.Back to article page