Activation of p53-mediated transcription by HIPK2 overexpression. 293 (A), HeLa (B) and Saos2 (C) cells were cotransfected with a p53-luciferase reporter construct and the indicated HIPK2 constructs or vector. Results represent the mean of three independent experiments. Bars, SD. (D) Western analysis of transient overexpression of Flag-tagged wildtype HIPK2 in HeLa cells (right lane) compared to vector-transfected control cells (left lane) with an anti-Flag antibody.