Table 4 Effects of substitutions of Lys61 on translational repression. The numbers shown are the relative β-galactosidase activities produced by bacteria containing the indicated plasmid constructs.
From: Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer
p2CTdl-13 | 3 |
pUCter3 | 100 |
2K61A | 82 |
2K61F | 87 |
2K61M | 60 |
2K61W | 21 |
2K61Y | 19 |
K61A-WT | 32 |
K61F-WT | 25 |
K61M-WT | 19 |
K61W-WT | 13 |
K61Y-WT | 20 |
K61A-Y85H | 20 |
K61F-Y85H | 24 |
K61M-Y85H | 33 |
K61W-Y85H | 25 |
K61Y-Y85H | 37 |
K61A-T91I | 77 |
K61F-T91I | 26 |
K61M-T91I | 44 |
K61W-T91I | 66 |
K61Y-T91I | 65 |