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Table 3 Effects of substitutions of Thr59 on translational repression. The numbers shown are the relative β-galactosidase activities produced by bacteria containing the indicated plasmid constructs.

From: Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer

p2CTdl-13

2

pUCter3

100

2T59A

ND

2T59F

10

2T59I

ND

2T59L

19

2T59N

8

2T59W

11

2T59Y

11

T59A-WT

3

T59F-WT

2

T59I-WT

2

T59L-WT

3

T59N-WT

ND

T59W-WT

3

T59Y-WT

2

T59A-Y85H

3

T59F-Y85H

3

T59I-Y85H

2

T59L-Y85H

2

T59N-Y85H

ND

T59W-Y85H

2

T59Y-Y85H

2

T59A-T91I

15

T59F-T91I

14

T59I-T91I

12

T59L-T91I

17

T59N-T91I

ND

T59W-T91I

15

T59-YT91I

16