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Figure 1 | BMC Molecular Biology

Figure 1

From: Coordinate regulation of RARgamma2, TBP, and TAFII135 by targeted proteolysis during retinoic acid-induced differentiation of F9 embryonal carcinoma cells

Figure 1

A. Depletion of TBP and TAFII135 in extracts from differentiated F9 cells. Replica plates of cells were treated with T-RA (or vehicle, right panel) at day 0 and extracts were subsequently prepared form one 10 cm plate at the day indicated above each lane. 10 μg of each cell extract were then subjected to SDS-PAGE transferred to nitrocellulose membranes and the presence of TBP, and TAFIIs was detected using the described antibodies. The locations of the detected proteins are indicated to the left. B. The extracts from cells differentiated for 0-9 days used in panel A were subjected to SDS-PAGE and stained with Coomasie Brilliant Blue. C. Western blot with anti-TBP and TAFII135 antibodies performed on serial dilutions of an extract from undifferentiated F9 cells were compared with an undiluted extract from 7 day differentiated cells. Lanes 1-5 contain 20, 10, 4, 2, and 1 μg of undifferentiated cell extract and lane 6, 20 μg of differentiated cell extract. D. Immunoblotting shows that TAFII135 and TBP are selectively depleted in F9 cell nuclear extracts. E. Extracts were prepared from F9 cells differentiated by the addition of T-RA and bt2cAMP for the number of days indicated above each lane.

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