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Table 2 Readthrough efficiencies from the mutant constructs

From: UAG readthrough in mammalian cells: Effect of upstream and downstream stop codon contexts reveal different signals

Test Codon Readthrough Standard
Sequnces Frequency Efficiency Deviation
CAGGLN UAG CAA 3.41% 0.77% 0.08%
CAAGLN UAG CAA 1.18% 2.01% 0.80%
CAUHIS UAG CAA 0.97% 0.06% 0.03%
CACHIS UAG CAA 1.50% 0.30% 0.18%
GGGGLY UAG CAA 1.59% 0.18% 0.08%
GGAGLY UAG CAA 1.80% 0.49% 0.23%
GGUGLY UAG CAA 1.21% 0.10% 0.04%
GGCGLY UAG CAA 2.31% 0.16% 0.08%
CAA UAG CAG   0.43% 0.05%
CAA UAG CAA   2.01% 0.80%
CAA UAG CAU   0.07% 0.04%
CAA UAG CAC   0.066% 0.05%
CAA UAG GGG   0.022% 0.01%
CAA UAG GGA   0.028% 0.01%
CAA UAG GGU   0.033% 0.01%
CAA UAG GGC   0.053% 0.03%
  1. (a) The last amino acid inserted before the stop is indicated together with the codon frequency compiled from Mus musculus [20]).