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Table 2 Readthrough efficiencies from the mutant constructs

From: UAG readthrough in mammalian cells: Effect of upstream and downstream stop codon contexts reveal different signals

Test

Codon

Readthrough

Standard

Sequnces

Frequency

Efficiency

Deviation

CAGGLN UAG CAA

3.41%

0.77%

0.08%

CAAGLN UAG CAA

1.18%

2.01%

0.80%

CAUHIS UAG CAA

0.97%

0.06%

0.03%

CACHIS UAG CAA

1.50%

0.30%

0.18%

GGGGLY UAG CAA

1.59%

0.18%

0.08%

GGAGLY UAG CAA

1.80%

0.49%

0.23%

GGUGLY UAG CAA

1.21%

0.10%

0.04%

GGCGLY UAG CAA

2.31%

0.16%

0.08%

CAA UAG CAG

 

0.43%

0.05%

CAA UAG CAA

 

2.01%

0.80%

CAA UAG CAU

 

0.07%

0.04%

CAA UAG CAC

 

0.066%

0.05%

CAA UAG GGG

 

0.022%

0.01%

CAA UAG GGA

 

0.028%

0.01%

CAA UAG GGU

 

0.033%

0.01%

CAA UAG GGC

 

0.053%

0.03%

  1. (a) The last amino acid inserted before the stop is indicated together with the codon frequency compiled from Mus musculus [20]).