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Figure 1 | BMC Molecular Biology

Figure 1

From: UAG readthrough in mammalian cells: Effect of upstream and downstream stop codon contexts reveal different signals

Figure 1

Description of the pRSVL74 vector and of the cloning strategy. The sequence of the 5' end of the luc gene coding sequence is shown with the common sequence of the inserted oligonucleotide containing the stop with its surrounding context. This sequence is inserted in place of the boxed "T" in the luc gene sequence. For each test construct, a control with the same sequence where a CAG replaced the TAG stop codon was used to evaluate the luciferase specific molecular activity.

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