Table 2 Strains and plasmids
From: A human RNA polymerase II subunit is encoded by a recently generated multigene family
Strains | Genotype |
|---|---|
Yeast WY-11 | # MATa/MATα ura3-52 his3-Δ200 leu2-3, leu2-112 lys2-Δ201 ade2-101 RPB11/rpb11-Δ1::HIS[39] |
Yeast YGVS-074 | # MATa/MATα ura3-52 his3-Δ200 leu2-3, leu2-112 lys2-Δ201 ade2-101 RPB11/rpb11-Δ1::HIS 3trp1-Δ63 |
Yeast YGVS-072 | # MATa ura3-52 his3-Δ200 leu2-3, leu2-112 lys2-Δ201 ade2-101 trp1-Δ63 rpb11-Δ1::HIS3 [pRP11/8-RPB11] (Offspring of YGVS-074 used for complementation assays) |
Plasmids | Description |
pRP11/8-RPB11 | # URA3 CEN ARS ScRPB11 EcoRI/SacI into pRS416 [39] |
pBSK-hRPB11a-gen | # Partial Sau3AI genomic fragment in pBSK (Stratagene), containing exon 1 and 2 from hRPB11a gene |
pBSK-hRPB11b-gen | # Partial Sau3AI genomic fragment (19.6 kb) in pBSK (Stratagene), containing exons 1 to 4 from hRPB11b gene |
pBSK-mRPB11-gen1 | # Partial Sau3AI genomic fragment (16.5 kb) in pBSK (Stratagene), containing exons 1 to 4 from mRPB11 gene |
pBSK-mRPB11-gen2 | # Partial Sau3AI genomic fragment (17.7 kb) in pBSK (Stratagene), containing exons 1 to 4 from mRPB11 gene |
pBSK-hRPB11a | # RT-PCR cloning of hRPB11a CDS in pBSK. The CDS can be excised using the unique Nhe I and Spe I sites |
pCRII-hRPB11bα | # RT-PCR cloning of hRPB11bα CDS in pCRII (Invitrogen). The CDS can be excised using Nhe I and Spe l |
pCRII-hRPB11bβ | # RT-PCR cloning of hRPB11bβ CDS in pCRII. The CDS can be excised using the flanking EcoRI sites |
pCRII-ScRPB11 | # PCR cloning of ScRPB11 CDS from pRP11/8-RPB11 in pCRII. The CDS can be excised using Nhe I and Spe l |
pGEN | # 2μORI, TRP1, PGK promoter [4] |
pGEN-ScRPB11 | # Cloning of the Eco RI fragment of pCRII-ScRPB11 into the Eco RI site of pGEN |
pGEN-hRPB11a | # Cloning of the Nhe I-Xba I fragment of pBSK-hRPB11a into the Nhe I site of pGEN |
pGEN-hRPB11bα | # Cloning of the Nhe I-Spe I fragment of pCRII-hRPB11bα into the Nhe I site of pGEN |
pGEN-hRPB11bβ | # Cloning of the Eco RI fragment of pCRII-hRPB11bβ into the Eco RI site of pGEN |
pVL1393-hRPB11a | # Cloning of the Nhe I-Xba I fragment of pBSK-hRPB11a into the Xba I site of pVL1393 (PharMingen) |
pVL1393-hRPB11bα | # Cloning of the Nhe I-Spe I fragment of pCRII-hRPB11bα into the Xba I site of pVL1393 (PharMingen) |
pVL1393-hRPB11bβ | # Cloning of the Nhe I-Spe I fragment of pCRII-hRPB11bβ into the Xba I site of pVL1393 (PharMingen) |