Structure of mRPB11 gene, mRNA and protein. A) Comparison of the structures of hRPB11a and mRPB11 genomic sequences. Horizontal lines represent the genomic sequences. The identified exons are indicated by boxes. The conserved 5' sequences encompassing exons 1–3 are in black, as in fig. 1A. The homologies between the mouse and human sequences which are restricted to the exons are indicated. B) Structure of mRPB11 mRNA. The exons are indicated by boxes. The carets represent the spliced introns whith sizes (bp). The 5' and 3' untranslated regions are shown as open boxes. The size (bp) of the coding sequence (CDS) present in each exon is indicated below. C) Amino acid sequences of mRPB11 polypeptide. The translated CDS of the mRNA identified for the mRPB11 and hRPB11a genes shown above are aligned with their identity and size (aminoacids) indicated on the left and right, respectively. The limits of the exons encoding each part of the sequence are indicated by brackets, with the corresponding exon numbers indicated above. The sequence of mRPB11 being taken as a reference, is aligned with hRPB11a, complete identity is indicated by the uninterrupted series of – symbols. D) Genomic localisation of mRPB11. A representative metaphase that has been simultaneously hybridised with the pBSK-mRPB11-gen1 and pBSK-mRPB11-gen2 derived fluorescent probes, respectively green and red, is shown. The whitearrow heads point to the position of the specifically bound loci. The hybridised chromosome is identified in the bottom of the figure, 1: both pBSK-mRPB11-gen1 and 2 probes are visualised, 2: only pBSK-mRPB11-gen1 probe is visualised, 3: only pBSK-mRPB11 gen2 probe is visualised, 4: chromosome staining.