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Table 4 Ranking of HKG stability by public algorithms

From: Validation of housekeeping gene and impact on normalized gene expression in clear cell Renal Cell Carcinoma: critical reassessment of YBX3/ZONAB/CSDA expression

Ranking

Ref finder

Ge norm

Norm finder

Best keeper

delta Ct

1

RPS13 (1.68)

PPIA/RPS13 (0.600)

RPL27 (0.244)

PPIA (1.074)

RPL27 (1.82)

2

PPIA (1.86)

RPL27 (0.883)

RPS13 (1.252)

RPS13 (1.202)

RPS13 (1.97)

3

RPL27 (1.86)

RP2 (1.038)

RP2 (1.276)

b-Act (1.250)

PPIA (2.01)

4

RP2 (3.94)

b-Act (1.206)

PPIA (1.387)

RPL27 (1.286)

RP2 (2.07)

5

b-Act (5.01)

18S (1.776)

18S (1.885)

RP2 (1.520)

18S (2.51)

6

18S (5.48)

B2M (2.084)

B2M (2.198)

18S (2.367)

b-Act (2.52)

7

B2M (6.74)

GAPDH (2.343)

b-Act (2.206)

B2M (2.472)

B2M (2.72)

8

GAPDH (8.00)

 

GAPDH (2.844)

GAPDH (3.161)

GAPDH (3.12)

  1. Each HKG (or pair of HKG) is ranked according to the indicated algorithms (respective stability scores indicated in brackets) and RefFinder assigned a final rank (geometric mean of stability scores for each gene). Lower values indicate more stable genes. Note that (i) PPIA and RPS13 rank at the top of 3 out of 5 algorithms; (ii) GAPDH and B2M are worst candidates; and (iii) beta-Actin ranks intermediate.