Table 2 List and characteristics of primers used in real-time PCR experiments
Target | Full name [mRNA NCBI accession ID] | Primer sequence (5′ to 3′) | Amplicon size (bp) | Slope | r2 | Eff |
|---|---|---|---|---|---|---|
β -Act | Actin beta [NM_001101.3] | F: AGGCCAACCGCGAGAAGATGACC | 332 | -3.486 | 0.998 | 1.94 |
R: GAAGTCCAGGGCGACGTAGCAC | ||||||
GAPDH | glyceraldehyde-3-phosphate dehydrogenase [NM_002046.4] | F: TTCTTTTGCGTCGCCAGCCGA | 96 | -3.402 | 0.998 | 1.97 |
R: GTGACCAGGCGCCCAATACGA | ||||||
18S | 18S ribosomal RNA [NR_003286.2] | F: GGCGCCCCCTCGATGCTCTTAG | 89 | -3.488 | 1 | 1.94 |
R: GCTCGGGCCTGCTTTGAACACTCT | ||||||
B2M | beta-2-microglobulin [NM_004048.2] | F: TGCCTGCCGTGTGAACCATGT | 97 | -3.319 | 0.997 | 2.00 |
R: TGCGGCATCTTCAAACCTCCATGA | ||||||
RP2 | polymerase (RNA) II (DNA directed) polypeptide A [NM_000937.4] | F: TCCCATGGGTGGAATCTCTCCTGC | 162 | -3.767 | 0.995 | 1.84 |
R: GAGTAACCTGGGCTGAAGCCGC | ||||||
PPIA | peptidylprolyl isomerase A (cyclophilin A) [NM_021130.3] | F: ACCGCCGAGGAAAACCGTGTA | 129 | -3.217 | 0.999 | 2.05 |
R: TGCTGTCTTTGGGACCTTGTCTGC | ||||||
RPL27 | ribosomal protein L27 [NM_000988.3] | F: TGGTAGGGCCGGGTGGTTGC | 185 | -3.203 | 0.997 | 2.05 |
R: ACTTTGCGGGGGTAGCGGTC | ||||||
RPS13 | ribosomal protein S13 [NM_001017.2] | F: TCGGCTTTACCCTATCGACGCAG | 153 | -3.429 | 0.999 | 1.96 |
R: ACGTACTTGTGCAACACCATGTGA | ||||||
YBX3 + Ψ | Y box binding protein 3 [NM_003651.4/NM_001145426.1] | F: CGG TTCATCGAAATCCAACT | 166 | -3.519 | 0.999 | 1.92 |
R: TAATTGTAGGGACGCCGGTA | ||||||
YBX3- Ψ | Y box binding protein 3 [NM_003651.4/NM_001145426.1] | F: CCACCAAAGTCCTTGGCACTGTC | 240 | -3.452 | 0.998 | 1.95 |
R: TC CCT TCCACAGGAACTCCAT CCG | ||||||
VEGFa | vascular endothelial growth factor A [NM_001025366.2] | F: AGAAACCACGCTGCCGCCAC | 118 | -3.191 | 0.993 | 2.06 |
R : GTCTCGCCCTCCGGACCCAA | ||||||
c-Myc | v-myc myelocytomatosis viral oncogene homolog [NM_002467.4] | F: TACAACACCCGAGCAAGGAC | 189 | -3.2 | 0.998 | 2.05 |
R: AGCTAACGTTGAGGGGCATC | ||||||
CycD1 | cyclin D1 [NM_053056.2] | F: CGCCCCACCCCTCCAG | 221 | -3.136 | 0.997 | 2.08 |
R: CCGCCCAGACCCTCAGACT | ||||||
PCNA | proliferating cell nuclear antigen [NM_002592.2] | F: GTAGTAAAGATGCCTTCTGGTG | 190 | -3.425 | 0.997 | 1.96 |
R: TCTCTATGGTAACAGCTTCCTC |