Figure 2
The α-globin promoter contains many consensus KLF3 binding sites. The human HBA2 (A) and mouse Hba-a2 (B) α-globin proximal promoter sequences, immediately 5′ to the transcriptional start site, were inspected for consensus binding sites, conforming to the sequence 5′-NCN CNC CCN-3′. The position and direction of binding sites are indicated by grey arrows. The sequences used in the design of probes for electrophoretic mobility shift assays are shown by black arrows. Also indicated are CAAT and TATA boxes. Sequences are numbered with respect to the transcription start site at +1.