Template
|
Reaction
|
Signal (RLU average)
|
% RNA synthesis*
|
% RNA synthesis
|
---|
TATA mutant vs. standard**
|
---|
HS-DNA_std
|
Positive
|
1306740
|
100% ± 3%
|
100% ± 3%
|
Negative
|
9768
| | |
α-amanitin 200 nM
|
161585
|
12% ± 1%
| |
HS-DNA_mut
|
Positive
|
985650
|
100% ± 10%
|
75% ± 7%
|
Negative
|
11795
| | |
α-amanitin 200 nM
|
130255
|
12% ± 1%
| |
- Transcription reactions were performed using the standard DNA template (HS-DNA_std) or a TATA-box mutated template (HS-DNA_mut). Transcription efficacy was determined by QuantiGene quantification.
- *Compared to respective average positive control, less the respective negative controls.
- **Compared to average positive control on standard template, less the respective negative controls.