Figure 7
From: Fidelity of end joining in mammalian episomes and the impact of Metnase on joint processing
Characterization of effect of Metnase on end-processing. (A) Representation of deleted clones obtained from Met-T-HO-CAT cells and T-HO-CAT cells after NotI digestion of rescued plasmid population at 6 h time point post-infection. (B) Size distribution of deleted clones obtained from the two different cell lines. (C) Cartoon displaying position of different restriction enzymes on HO-CAT episome used for various digestions and southern blot. (D) Southern blot for pRep4-HO-CAT fragments recovered at given time points PI from cell lines in presence or absence of Metnase, after appropriate restriction digestion (see the description in text). N.I. denotes the episomes recovered from non-infected cells. Different arrow positions show processed episomal products. 11Kb size arrow denotes the singly-cut plasmid. M denotes the molecular weight marker, that is NEB 1 kb ladder labeled with PNK and γATP32.