Figure 6
From: Fidelity of end joining in mammalian episomes and the impact of Metnase on joint processing
Metnase promotes erroneous end-processing. (A) Immunoblot showing stable Metnase overexpression in Met -THO-CAT cells along with endogenous level of Metnase in 3-HO-CAT cells. Arrow marks the correct size band for Metnase in the indicated cells. (B) qPCR analysis of kinetics of episomal cleavage and repair in 293 T cells (without Metnase) and Met-T-HO-CAT cells (expressing Metnase) as described in Figure 1B &1C. qPCR values are represented as (CAT/AMP)% over control. (C) Replica plating assay conducted for indicated time points in three different cell lines with varying Metnase expression. (D) Table showing average replica plating efficiency obtained from 3 different time points for each cell line and from three independent experiments.