Figure 3

Molecular analysis of random integration clones derived from transformation with no-ITRs-containing fragment. Southern blot analysis of genomic DNA from fourteen URA3⁺LYS2⁺ yeast clones derived from transformation of RSY12 yeast strain with pAAVLUL digested with Xba I. Digestion with Xba I produces a fragment without ITR as described in Figure 1B. Genomic DNA was digested with Ase I that does not cut in the sequence between the ITRs. The numbers above the filters indicate the clones. Bands were detected using the URA3 probe.