Paneth cells are decreased in Gata6ΔIE and DKO mice. (A) Immunostaining in ileum for CRS4C (first row) and MUC2 (third row), and chemical staining using the PAS reaction (second row). (B) Quantification of CRS4C-positive cells in each group. Sections of ileum were stained for CRS4C, and the number of positive cells/crypt cross section was determined as described in Methods. (C) Lyz and Muc2 mRNA abundance in each group. Gata6Δ IE and DKO mice had significantly lower numbers of CRS4C-positive cells and Lyz mRNA abundance than Ctl mice, consistent with a decrease in mature Paneth cells.