Enteroendocrine cell allocation is decreased in Gata6ΔIE and DKO mice. (A) Quantification of CHGA-positive cells in each group. Sections of ileum were stained for CHGA, and the number of positive cells/1000 epithelial cells was determined as described in Methods. (B) Chga and Neurog3 mRNA abundance in each group. Gata6Δ IE and DKO mice had generally lower numbers of CHGA-positive cells and Chga and Neurog3 mRNA abundances than Ctl mice, consistent with a decrease in enteroendocrine lineage allocation.