Skip to main content
Figure 1 | BMC Molecular Biology

Figure 1

From: Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells

Figure 1

Expression of glycosyltransferase and glycosidase genes (glycogenes) involved in the biosynthesis of Fs antigen. (A) Schematic presentation of the biosynthesis pathway of Fs pentasaccharide glycosphingolipid from glycosylceramide and of the responsible glycosyltransferases (downward arrows) and glycosidases (upward arrows): 1. glycosylceramide; 2. lactosylceramide; 3. globotriaosylceramide (Gb3, Pk); 4. globoside (P antigen, Gb4); 5. Forssman (Fs, IV3GalNAcα-Gb4Cer). Filled circles (glucose, Glc); open circles (galactose, Gal); squares (N-acetylgalactosamine, GalNAc). B) Heat map with the color key representing normalized (ΔCq) and clustering of glycogene expression (rows) among the investigated cell lines (columns). Color key indicates relative level of expression from high (white) to low (black). Dendrogram shows the result of the hierarchical clustering calculation for the glycogene expression. (C) Normalized expression of GBGT1 and NAGA; Mean ± SD (three independent experiments) in the presented cell lines. A high ΔCq value indicates low level of normalized gene expression. (D) Western blot analysis (autoradiograph and corresponding quantitative analysis) showing GBGT1 protein expression in the presented cell lines (actin is the sample loading control).

Back to article page