Figure 4From: Establishment of a quantitative PCR system for discriminating chitinase-like proteins: catalytically inactive breast regression protein-39 and Ym1 are constitutive genes in mouse lungEvaluation of the CLPs primers suitability for qPCR. The PCR primers were evaluated based on whether they gave one melting temperature (A-C) and a single PCR product on a 10% polyacrylamide gel (D). The y axis was expressed as first derivative of the fluorescence intensity as a function of temperature (A-C). To verify specificity, the dissociation curves of the PCR products for the three genes were generated using a mouse tissue cDNA mixture. The PCR products were analyzed on a 10% polyacrylamide gel, followed by ethidium bromide staining (D).Back to article page