Figure 2From: Establishment of a quantitative PCR system for discriminating chitinase-like proteins: catalytically inactive breast regression protein-39 and Ym1 are constitutive genes in mouse lungPreparation and validation of the mouse Refs/CLPs standard DNA with pGEM-T Easy. (A) Schematic representation of the mouse Refs/CLPs standard DNA cloning into pGEM-T Easy vector. (B) The mouse Refs/CLPs standard DNA with pGEM-T Easy was PCR-amplified from the plasmid DNA using the BRP-39 forward (blue arrow) and Ym1 reverse (red arrow) primers. As described above, we amplified the Ym1 and Ym2 cDNAs from this standard DNA using the Ym1 (C) and Ym2 (D) primers and these PCR products were analyzed using 10% polyacrylamide gel electrophoresis (E). The y axis was expressed as first derivative of the fluorescence as a function of temperature (C and D).Back to article page