Figure 4

Wnt4 expression correlates with the maintenance of the epithelial state. A) Effect of Wnt4 silencing on EMT of thyroid cells. qRT-PCR analysis was performed on total RNA prepared from FRTL-5 cells transfected with siWnt4 or siCtrl-. The expression of E-cad, α-SMA and VIM was measured; B) Effect of Wnt4 overexpression on EMT of thyroid cells. qRT-PCR analysis was performed on total RNA prepared from FRTL-5-Wnt4 clones 31 and 35 and from FRTL-5 stable transfected with the empty vector. The expression of E-cad, α-SMA and Vim was measured. Data are expressed as the mean ± SD (P < 0,05). On the right, protein levels were evaluated by immunoblotting analysis using Wnt4, E-cad and VIM antibodies. The hybridization with GAPDH assessed the protein uniform loading integrity. Fold represents the quantification of protein levels normalized with GAPDH.