Figure 2

In vivo tagging of truncated ribosomal protein L27 by E. coli tmRNA(H8) and M. tuberculosis tmRNA(H6). E. coli IW764 cells were transformed with plasmids pWOW-Δ/E (lane 1), pWOW-E/E (lane 2), pWOW-M/E (lane 3), pWOW-Δ/M (lane 4), pWOW-M/M (lane 5) and pWOW-E/M (lane 6). Lysates of IPTG-induced E. coli IW764 cells harboring plasmid pWOW derivatives were fractionated on a 12.5% SDS-polyacrylamide gel. Tagged proteins were detected by staining the gel with Coomassie Blue. L27 and L27* denote truncated and tagged ribosomal protein L27, respectively. Positions of 6.0 and 14.4 kDa molecular markers are shown on the left side of the gel image.