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Table 2 Results from PCR based genotyping of targeted integration into F. graminearum and F. avenaceum

From: Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system

 

No. tested

Class 1 (NHEJ/NHEJ)

Class 2 (NHEJ/HR)

Class 3 (HR/HR)

Gene targeting efficiency (%)

F. graminearum PKS3

104

1

15

88

84.6%

F. avenaceum PKS3

100

10

16

74

74.0%

Other loci in F. avenaceum

Deletion of 13 PKSs

129

13

23

93

72.1%

In locus overexpression 12 PKSs

116

20

1

95

81.9%

  1. NHEJ = non-homologous end joining; HR = homologous recombination. Class 1 transformants represent random integration events; Class 2 represents transformants where the T-DNA has integration by HR at one end and via an unknown mechanism at the opposite end (likely NHEJ); Class 3 transformants represent the desired gene targeting event where both ends have integrated by HR. The number for ‘other loci’ in F. avenaceum represents the average for all 13 deletions and 12 in locus overexpression experiments; see Additional file 1: Table S7 for results for the individual locus.