Table 1 Standard test primers for the analysis of the obtained transformants
Primer name | Sequence (5′ to 3′) | Amplicon | Target |
|---|---|---|---|
Primers for testing of the selection markers | |||
HygR-T-F | AGCTGCGCCGATGGTTTCTACAA | 588 bp | Test for marker gene |
HygR-T-R | GCGCGTCTGCTGCTCCATACAA | ||
GenR-T-F | AGCCCATTCGCCGCCAAGTTCT | 480 bp | |
GenR-T-R | GCAGCTGTGCTCGACGTTGTCA | ||
BAR-T-F | TCAGATCTCGGTGACGGGCA | 552 bp | |
BAR-T-R | ATGAGCCCAGAACGACGCC | ||
Generic primers for testing targeted integration | |||
RF-1 (HygR constructs) | 5′-AAATTTTGTGCTCACCGCCTGGAC | * | T-DNA |
RF-2 (HygR constructs) | 5′-TCTCCTTGCATGCACCATTCCTTG | * | T-DNA |
RF-3 (PgpdA promoter) | 5′-TTGCGTCAGTCCAACATTTGTTGCCA | * | T-DNA |
RF-7 (GenR constructs) | 5′-CTTTGCGCCCTCCCACACAT | * | T-DNA |
RF-6 (GenR constructs) | 5′-TCAGACACTCTAGTTGTTGACCCCT | * | T-DNA |
RF-8 (BarR constructs) | 5′-CTGCACTTTTATGCGGTCACACA | * | T-DNA |
RF-9 (BarR constructs) | 5′-CCTAGGCCACACCTCACCTTATTCT | * | T-DNA |