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Figure 1 | BMC Molecular Biology

Figure 1

From: Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system

Figure 1

The different DNA fragments (=bricks) in the USER-Brick vector system. The ends of the Bricks are colour coded based on which overhangs that are compatible for fusion. Top panel: The core USER-Brick includes backbones, selection markers, promoters and fluorescent marker fragments. Centre panel: The placement of the different types of PCR amplicons in relation to the gene of interest. Bottom panel: Sequences of the 5′ overhang found on the primers for amplifying the different USER-Bricks in the two panels above.

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BMC Molecular Biology

ISSN: 1471-2199

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