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Figure 3 | BMC Molecular Biology

Figure 3

From: Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases

Figure 3

Characterization of mutagenic events promoted by the HtH and TtH nuclease architectures in mammalian cells. (A) Alignment of the WT genomic sequence and predominant deletion events induced by the HtH architecture at two different endogenous loci. FokI::TALE scaffold binding sites are represented by capital letters. (B) Same as (A) but for the TtH architecture at the three different endogenous loci. The targeted sequence is colored with respect to the scaffold binding site considered (blue: FokI::TALE, red: TALE::FokI). The position 0 is underlined.

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