Figure 4

The expression of DZIP1 and its mRNA targets is affected by blockade of the Hh pathway. (A-D) Indirect immunofluorescence staining was used to detect DZIP1 (red – alexa546), and nuclei were counterstained with DAPI (blue). (A-B) HeLa cells not treated with cyclopamine. (C-D) HeLa cells treated with 300 nM cyclopamine for 24 h. (E-H) Quantitative RT-PCR analysis of DZIP1, GLI1, BRD8 and PTCH1 mRNA levels in HeLa cells treated with cyclopamine for 24, 48 or 72 h. GAPDH was used as an internal housekeeping gene control. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Scale bar: 10 μm.