Table 1 Reference genes for qPCR
Gene | Gene | NCBI reference sequence | Forward sequence | Cellular function |
|---|---|---|---|---|
18SrRNA | 18S ribosomal RNA | NR_003286.2 | F = 5’-GTAACCCGTTGAACCCCATT-3’ | Small subunit of cytoplasmic ribosomes |
R = 5’-CCATCCAATCGGTAGTAGCG-3’ | ||||
ACTB | beta-actin | NM_001101.3 | F = 5’-CCTTTTTGTCCCCCAACTTGA-3’ | Cytoskeleton structural protein; motility, cytokinesis |
R = 5’-TGGCTGCCTCCACCCA-3’ | ||||
EF1a | elongation factor-1 alpha | NM_001402.5 | F = 5’-AGGTGATTATCCTGAACCATCC-3’ | Translation |
R = 5’-AAAGGTGGATAGTCTGAGAAGC-3’ | ||||
GAPDH | glyceraldehyde 3-phosphate dehydrogenase | NM_002046.4 | F = 5’-CAACGGATTTGGTCGTATTGG-3’ | Oxido-reductase in glycolysis and gluconeogenesis, transcription activation, apoptosis |
R = 5’-GCAACAATATCCACTTTACCAGAGTTAA-3’ | ||||
GUSB | beta-glucuronidase | NM_000181.3 | F = 5’-CTCATTTGGAATTTTGCCGATT-3’ | Catalyzing hydrolysis of B-D-glucuronic acid |
R = 5’-CCGAGTGAAGATCCCCTTTTTA-3’ | ||||
PPIA | peptidyl prolyl isomerase A | NM_021130.3 | F = 5’-TCCTGGCATCTTGTCCATG-3’ | Protein folding |
R = 5’-CCATCCAACCACTCAGTCTTG-3’ | ||||
RPL13 | ribosomal protein L13a | NM_012423.3 | F = 5’-CATAGGAAGCTGGGAGCAAG-3’ | Structural component of 60S ribosomal subunit |
R = 5’-GCCCTCCAATCAGTCTTCTG-3’ | ||||
TBP | TATA-binding protein | NM_003194.4 | F = 5’-TGCACAGGAGCCAAGAGTGAA-3’ | RNA polymerase II transcription factor |
R = 5’-CACATCACAGCTCCCCACCA-3’ | ||||
YWHAZ | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | NM_001135702.1 | F = 5’-ACTTTTGGTACATTGTGGCTTCAA-3’ | Signal transduction |
R = 5’-CCGCCAGGACAAACCAGTAT-3’ |