Effect of miR-378 overexpression on C2C12 differentiation. A-C) Creatine kinase (A) and Alp (B) activity in and calcium deposition (C) by C2C12-pMirn0 (light bars) and C2C12-pMirn378 (dark bars) cells after 6 (A-B) or 10 (C) days of culture in the presence or absence of 300 ng/ml BMP2. A) The mean values +/− SD of two biological replicates, whereby each measurement was made in duplicate, are shown. B) Mean values +/− SD of three independent experiments with biological duplicates each. For each independent experiment, Alp activity values were normalized to the average of the C2C12-pMirn0 samples treated with BMP2. C) Data shows calcium deposition in 24-well plates and is representative of 2 independent experiments. Mean values +/− SD of two biological replicates in one experiment, whereby each measurement was made in duplicate, are shown. *p < 0.05 when compared to the C2C12-pMirn0 sample with the same treatment. AU: arbitrary units.