Effect of miR-378 overexpression on C2C12 bone transcription program: downregulation. A) Venn diagram representing the number of probes on the microarray that are significantly (as defined in Methods) lower expressed in C2C12-pMirn378 cells than in control C2C12-pMirn0 cells at each time point during differentiation in the presence of 300 ng/ml BMP2. B) Enriched (p < 0.01) GO terms (in biological processes category) within the set of probes that are significantly lower expressed in C2C12-pMirn378 versus C2C12-pMirn0 cells at at least two consecutive time points during differentiation in the presence of 300 ng/ml BMP2. C) Microarray profiles of representative genes that are expressed at significantly lower levels in C2C12-pMirn378 cells than in C2C12-pMirn0 cells at at least two consecutive time points during differentiation in the presence of BMP2 (light bullets: C2C12-pMirn0, dark bullets: C2C12-pMirn378, squares: treatment with BMP2, circles: treatment without BMP2); Myog, troponin T2, cardiac (Tnnt2; 1418726_a_at), actin alpha cardiac muscle 1 (Actc1; 1415927_at), wingless-related MMTV integration site 5a (Wnt5a; 1436791_at) and 10a (Wnt10a; 1460657_at). For Myog, Tnnt2 and Actc1, expression levels are only shown for cells treated with BMP2. Mean expression values +/− SD from triplicate microarray experiments are shown for all data points. When the error bar is not visible, the SD falls within the printed data point. All SD values are, however, listed in Additional file 2. AEV = average expression value; *significantly different expression in C2C12-pMirn378 compared to control C2C12-pMirn0 at the same time point and treatment (see Methods for definition).