Skip to main content
Figure 2 | BMC Molecular Biology

Figure 2

From: MicroRNA miR-378 promotes BMP2-induced osteogenic differentiation of mesenchymal progenitor cells

Figure 2

Effect of miR-378 overexpression on C2C12 muscle transcription program. A) Mature miR-378 levels in C2C12-pMirn0 (light bars) and C2C12-pMirn378 (dark bars) cells at indicated time points during treatment with or without 300 ng/ml BMP2. MiR-378 levels are expressed as a percentage of the control small, non-coding RNA snoRNA202. Mean values +/− SD of three biological replicates, whereby each measurement was made in duplicate, are shown. *p < 0.05 when compared to the C2C12-pMirn0 sample at the same time point and treatment. B-C) Venn diagrams representing the number of probes on the microarray that are significantly lower (B) or higher (C) expressed in C2C12-pMirn378 cells than in control C2C12-pMirn0 cells at each time point during differentiation in the absence of BMP2. D) Enriched (p < 0.01) GO terms within the set of probes that are significantly lower expressed in C2C12-pMirn378 versus C2C12-pMirn0 cells at at least two consecutive time points during differentiation in the absence of BMP2. E) mRNA expression profiles of the muscle transcription factors myogenin (Myog; 1419391_at) and myocyte enhancer factor 2C (Mef2c; 1421027_a_at) and other muscle marker genes muscle creatine kinase (Ckm; 1417614_at), the acetylcholine receptor subunit gamma (Chrng; 1449532_at) and the sarcomeric genes fast troponin C2 (Tnnc2; 1417464_at) and actinin alpha 3 (Actn3; 1418677_at) at indicated time points during differentiation of C2C12-pMirn0 (light bullets) and C2C12-pMirn378 (dark bullets) cells treated with (squares) or without (circles) 300 ng/ml BMP2 as revealed from microarray analysis. Mean expression values +/− SD from triplicate microarray experiments are shown for all data points. When the error bar is not visible, the SD falls within the printed data point. All SD values are, however, listed in Additional file 2. AEV = average expression value; *significantly different expression in C2C12-pMirn378 compared to control C2C12-pMirn0 at the same time point and treatment.

Back to article page