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Table 2 Detection of six selected genes by RT-qPCR from a total of 9 samples in the 1 and 10 cell category and 10 samples in the 5 cell category

From: Single cell transcriptomic analysis of prostate cancer cells

 

# of samples detected by RT-qPCR

Rank percentile (n = 38695 probes)

Gene

1-cell (n = 9)

5-cell (n = 10)

10-cell (n = 9)

1-cell (n = 9)

5-cell (n = 10)

10-cell (n = 9)

ACTB

7

7

9

100

100

100

GAPDH

8

10

9

94

94

90

YWHAZ

8

10

9

97

98

98

RPL13A

9

10

9

99

100

100

  1. Detection was considered as having a Cq of <30. Rank percentile represents the average Alexa Fluor® 3 intensity from all oligonucleotides on the array that passed quality control for a given gene with outliers excluded using Dixon’s Q test. n = number of samples analyzed.